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rmtim 1 fc  (R&D Systems)


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    Structured Review

    R&D Systems rmtim 1 fc
    Rmtim 1 Fc, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+kim+1+protein/Recombinant+Mouse+TIM-1%2FKIM-1%2FHAVCR+Isoform+A+Fc+Protein%2C+CF/bio_rxiv__64898__2025__12__22__696083-230-29-30
    Average 93 stars, based on 1 article reviews
    rmtim 1 fc - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Incubation:

    Article Title: Circulating KIM-1 levels for detection of pathologies associated with injury to, or cancer of, the kidney
    Article Snippet: KIM-1 Measurement Microbead-based assays for rodent and human plasma and serum KIM-1 were developed, and extensive validation of the assays were performed using previously described approaches.24 Urinary KIM-1 in rodents and humans was measured using microbead based assays as described previously.5,7,25,26 Capture antibodies (MAB 1817 for mouse, AF1750 for human [R&D Systems]), MARKE25 antibody for rat (developed at Brigham and Women's Hospital) were conjugated with COOH polystyrene beads (Bio-Rad) with an amine coupling kit (Bio-Rad) using N-hydroxysuccinamide-1-ethyl-3-(3-dimethylaminopropyl) carbodiimide chemistry according to the manufacturer's protocol. .. Approximately 6000 beads in 50 ml of sample diluent buffer (0.1M HEPES, 0.1M NaCl, 0.1% Tween-20, and 1% BSA; pH, 7.4; filter sterilized) were incubated with 30 ml of sample or recombinant KIM-1 protein (1817-TM-050-CF for mice, 1750-TM for humans [R&D systems]), and KIM-FC for rats [developed at Brigham and Women's Hospital]) for 45 minutes. .. After incubation, beads were washed three times with PBS with Tween and incubated with biotinylated anti-KIM-1 detection antibody (BAF 1817 for mice, BAF 1750 for humans [R&D Systems]) and MARKE-2 for rats [developed at Brigham and Women's Hospital]) for 30 minutes.

    Article Title: Blood Kidney Injury Molecule-1 Is a Biomarker of Acute and Chronic Kidney Injury and Predicts Progression to ESRD in Type I Diabetes
    Article Snippet: 5 , 7 , 25 , 26 Capture antibodies (MAB 1817 for mouse, AF1750 for human [R&D Systems]), MARKE 25 antibody for rat (developed at Brigham and Women’s Hospital) were conjugated with COOH polystyrene beads (Bio-Rad) with an amine coupling kit (Bio-Rad) using N-hydroxysuccinamide-1-ethyl-3-(3‐dimethylaminopropyl) carbodiimide chemistry according to the manufacturer’s protocol. .. Approximately 6000 beads in 50 μ l of sample diluent buffer (0.1M HEPES, 0.1M NaCl, 0.1% Tween-20, and 1% BSA; pH, 7.4; filter sterilized) were incubated with 30 μ l of sample or recombinant KIM-1 protein (1817-TM-050-CF for mice, 1750-TM for humans [R&D systems]), and KIM-FC for rats [developed at Brigham and Women’s Hospital]) for 45 minutes. .. After incubation, beads were washed three times with PBS with Tween and incubated with biotinylated anti–KIM-1 detection antibody (BAF 1817 for mice, BAF 1750 for humans [R&D Systems]) and MARKE-2 for rats [developed at Brigham and Women’s Hospital]) for 30 minutes.

    Article Title: Blood Kidney Injury Molecule-1 Is a Biomarker of Acute and Chronic Kidney Injury and Predicts Progression to ESRD in Type I Diabetes
    Article Snippet: Microbead-based assays for rodent and hu- man plasma and serum KIM-1 were devel- oped, and extensive validation of the assays were performed using previously described approaches.24 Urinary KIM-1 in rodents and humans was measured using microbead based assays as described previously.5,7,25,26 Capture antibodies (MAB 1817 for mouse, AF1750 for human [R&D Systems]), MARKE25 antibody for rat (developed at Brigham and Women’s Hospital) were con- jugated with COOH polystyrene beads (BioRad) with an amine coupling kit (Bio-Rad) using N-hydroxysuccinamide-1-ethyl-3-(3‐ dimethylaminopropyl) carbodiimide chemis- try according to the manufacturer’s protocol. .. Approximately 6000 beads in 50 ml of sample diluent buffer (0.1M HEPES, 0.1M NaCl, 0.1% Tween-20, and 1% BSA; pH, 7.4; filter sterilized) were incubated with 30 ml of sample or recombinant KIM-1 protein (1817-TM- 050-CF for mice, 1750-TM for humans [R&D systems]), and KIM-FC for rats [developed at Brigham and Women’s Hospital]) for 45 mi- nutes. .. After incubation, beads were washed three times with PBS with Tween and incu- bated with biotinylated anti–KIM-1 detection antibody (BAF 1817 for mice, BAF 1750 for humans [R&D Systems]) and MARKE-2 for rats [developed at Brigham and Women’s Hospital]) for 30 minutes.

    Recombinant:

    Article Title: Circulating KIM-1 levels for detection of pathologies associated with injury to, or cancer of, the kidney
    Article Snippet: KIM-1 Measurement Microbead-based assays for rodent and human plasma and serum KIM-1 were developed, and extensive validation of the assays were performed using previously described approaches.24 Urinary KIM-1 in rodents and humans was measured using microbead based assays as described previously.5,7,25,26 Capture antibodies (MAB 1817 for mouse, AF1750 for human [R&D Systems]), MARKE25 antibody for rat (developed at Brigham and Women's Hospital) were conjugated with COOH polystyrene beads (Bio-Rad) with an amine coupling kit (Bio-Rad) using N-hydroxysuccinamide-1-ethyl-3-(3-dimethylaminopropyl) carbodiimide chemistry according to the manufacturer's protocol. .. Approximately 6000 beads in 50 ml of sample diluent buffer (0.1M HEPES, 0.1M NaCl, 0.1% Tween-20, and 1% BSA; pH, 7.4; filter sterilized) were incubated with 30 ml of sample or recombinant KIM-1 protein (1817-TM-050-CF for mice, 1750-TM for humans [R&D systems]), and KIM-FC for rats [developed at Brigham and Women's Hospital]) for 45 minutes. .. After incubation, beads were washed three times with PBS with Tween and incubated with biotinylated anti-KIM-1 detection antibody (BAF 1817 for mice, BAF 1750 for humans [R&D Systems]) and MARKE-2 for rats [developed at Brigham and Women's Hospital]) for 30 minutes.

    Article Title: Blood Kidney Injury Molecule-1 Is a Biomarker of Acute and Chronic Kidney Injury and Predicts Progression to ESRD in Type I Diabetes
    Article Snippet: 5 , 7 , 25 , 26 Capture antibodies (MAB 1817 for mouse, AF1750 for human [R&D Systems]), MARKE 25 antibody for rat (developed at Brigham and Women’s Hospital) were conjugated with COOH polystyrene beads (Bio-Rad) with an amine coupling kit (Bio-Rad) using N-hydroxysuccinamide-1-ethyl-3-(3‐dimethylaminopropyl) carbodiimide chemistry according to the manufacturer’s protocol. .. Approximately 6000 beads in 50 μ l of sample diluent buffer (0.1M HEPES, 0.1M NaCl, 0.1% Tween-20, and 1% BSA; pH, 7.4; filter sterilized) were incubated with 30 μ l of sample or recombinant KIM-1 protein (1817-TM-050-CF for mice, 1750-TM for humans [R&D systems]), and KIM-FC for rats [developed at Brigham and Women’s Hospital]) for 45 minutes. .. After incubation, beads were washed three times with PBS with Tween and incubated with biotinylated anti–KIM-1 detection antibody (BAF 1817 for mice, BAF 1750 for humans [R&D Systems]) and MARKE-2 for rats [developed at Brigham and Women’s Hospital]) for 30 minutes.

    Article Title: Blood Kidney Injury Molecule-1 Is a Biomarker of Acute and Chronic Kidney Injury and Predicts Progression to ESRD in Type I Diabetes
    Article Snippet: Microbead-based assays for rodent and hu- man plasma and serum KIM-1 were devel- oped, and extensive validation of the assays were performed using previously described approaches.24 Urinary KIM-1 in rodents and humans was measured using microbead based assays as described previously.5,7,25,26 Capture antibodies (MAB 1817 for mouse, AF1750 for human [R&D Systems]), MARKE25 antibody for rat (developed at Brigham and Women’s Hospital) were con- jugated with COOH polystyrene beads (BioRad) with an amine coupling kit (Bio-Rad) using N-hydroxysuccinamide-1-ethyl-3-(3‐ dimethylaminopropyl) carbodiimide chemis- try according to the manufacturer’s protocol. .. Approximately 6000 beads in 50 ml of sample diluent buffer (0.1M HEPES, 0.1M NaCl, 0.1% Tween-20, and 1% BSA; pH, 7.4; filter sterilized) were incubated with 30 ml of sample or recombinant KIM-1 protein (1817-TM- 050-CF for mice, 1750-TM for humans [R&D systems]), and KIM-FC for rats [developed at Brigham and Women’s Hospital]) for 45 mi- nutes. .. After incubation, beads were washed three times with PBS with Tween and incu- bated with biotinylated anti–KIM-1 detection antibody (BAF 1817 for mice, BAF 1750 for humans [R&D Systems]) and MARKE-2 for rats [developed at Brigham and Women’s Hospital]) for 30 minutes.



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